Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers

Manik Chandra Barman; Srabone Sarker; Atikur Rahaman; Syed Ali Azmal; Jannatun Nime; Abdus Sattar; Akramul Haque; Shiekh Mohammad Abdul Matin; Abdul Hamid

doi:doi:10.11648/j.ijgg.20231101.14

Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers

Manik Chandra Barman, Srabone Sarker, Atikur Rahaman, Syed Ali Azmal, Jannatun Nime, Abdus Sattar, Akramul Haque, Shiekh Mohammad Abdul Matin, Abdul Hamid

Published in International Journal of Genetics and Genomics (Volume 11, Issue 1)

Received: 18 February 2023 Accepted: 7 March 2023 Published: 16 March 2023

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Abstract

Forty-eight crossbreed heifers were artificially inseminated within 10-20 hours after standing heat to evaluate the effects of different doses of hormones and age on reproductive performance synchronized with GnRH and PGF2α. On day 90 after artificial insemination, the conception rate was confirmed by rectal palpation. There were significant differences (P<0.05) in oesturs response in maximum doses (1.5 ml GnRH used in group III) of the hormone-treated group. The conception rate was significantly higher (P<0.05) in the highest treatment group (1.5 ml, 66.67%) than in low treatment groups (1 ml and 1.3 ml; 42.85% and 44.44%; respectively) in morning synchronization protocol (83.33%). The effect of different doses of the hormone on conception rates was statistical significance on the age of crossbreed heifers (P<0.05), time of initiation of ovsynch (P<0.05), time of insemination (P<0.05), semen placement (P<0.05), body condition score (P<0.05), retrieve to natural oesturs and subsequent pregnancy rate (P<0.05). On the other hand, there was no significant (P>0.05) effect on conception rate by the onset of oesturs and duration of oesturs. The highest oesturs rate and conception rate was observed at 75.00% and 66.67%; respectively by administration of maximum doses of the hormone of body condition score (3) 69.23% of crossbreed heifer. When 1.5 ml GnRH was used in group III, the conception rate was observed higher at 25-30 months of age (77.78%) which were inseminated in the site of the deep cervix (77.77%) with frozen-thaw semen by experience inseminator at 10-14 hours (75%) after the onset of oesturs and naturally retrieve of oesturs on conception rate (75%). It was concluded that the age of the heifers, doses of the hormone, Body Condition Score (BCS), time of initiation of synchronization protocol, time of AI, semen placement and retrieve to oesturs naturally were the most important factors to get maximum oesturs response and conception rate of crossbreed heifers. So, it may be suggested that to achieve the desired rate of oesturs and conception, the farmers should inseminate their crossbreed heifers (BCS, 3 out of 5) with maximum doses of hormone in the deep cervix by technically sound and skilled inseminator at 10-14 hours after onset of oesturs in morning synchronization protocol. GnRH and PGF2α analogs could successfully induce oesturs response and improve the fertility of a dairy herd. Therefore, ovsynch protocol for crossbreed heifers is needed for further work involving a large number of animals for a definite conclusion.

Published in	International Journal of Genetics and Genomics (Volume 11, Issue 1)
DOI	10.11648/j.ijgg.20231101.14
Page(s)	27-37
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Crossbred, Hormones, Oesturs Synchronization, AI & Conception Rate

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Manik Chandra Barman, Srabone Sarker, Atikur Rahaman, Syed Ali Azmal, Jannatun Nime, et al. (2023). Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers. International Journal of Genetics and Genomics, 11(1), 27-37. https://doi.org/10.11648/j.ijgg.20231101.14

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ACS Style

Manik Chandra Barman; Srabone Sarker; Atikur Rahaman; Syed Ali Azmal; Jannatun Nime, et al. Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers. Int. J. Genet. Genomics 2023, 11(1), 27-37. doi: 10.11648/j.ijgg.20231101.14

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AMA Style

Manik Chandra Barman, Srabone Sarker, Atikur Rahaman, Syed Ali Azmal, Jannatun Nime, et al. Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers. Int J Genet Genomics. 2023;11(1):27-37. doi: 10.11648/j.ijgg.20231101.14

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@article{10.11648/j.ijgg.20231101.14,
  author = {Manik Chandra Barman and Srabone Sarker and Atikur Rahaman and Syed Ali Azmal and Jannatun Nime and Abdus Sattar and Akramul Haque and Shiekh Mohammad Abdul Matin and Abdul Hamid},
  title = {Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {27-37},
  doi = {10.11648/j.ijgg.20231101.14},
  url = {https://doi.org/10.11648/j.ijgg.20231101.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.14},
  abstract = {Forty-eight crossbreed heifers were artificially inseminated within 10-20 hours after standing heat to evaluate the effects of different doses of hormones and age on reproductive performance synchronized with GnRH and PGF2α. On day 90 after artificial insemination, the conception rate was confirmed by rectal palpation. There were significant differences (PPPPPPPPP>0.05) effect on conception rate by the onset of oesturs and duration of oesturs. The highest oesturs rate and conception rate was observed at 75.00% and 66.67%; respectively by administration of maximum doses of the hormone of body condition score (3) 69.23% of crossbreed heifer. When 1.5 ml GnRH was used in group III, the conception rate was observed higher at 25-30 months of age (77.78%) which were inseminated in the site of the deep cervix (77.77%) with frozen-thaw semen by experience inseminator at 10-14 hours (75%) after the onset of oesturs and naturally retrieve of oesturs on conception rate (75%). It was concluded that the age of the heifers, doses of the hormone, Body Condition Score (BCS), time of initiation of synchronization protocol, time of AI, semen placement and retrieve to oesturs naturally were the most important factors to get maximum oesturs response and conception rate of crossbreed heifers. So, it may be suggested that to achieve the desired rate of oesturs and conception, the farmers should inseminate their crossbreed heifers (BCS, 3 out of 5) with maximum doses of hormone in the deep cervix by technically sound and skilled inseminator at 10-14 hours after onset of oesturs in morning synchronization protocol. GnRH and PGF2α analogs could successfully induce oesturs response and improve the fertility of a dairy herd. Therefore, ovsynch protocol for crossbreed heifers is needed for further work involving a large number of animals for a definite conclusion.},
 year = {2023}
}

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TY  - JOUR
T1  - Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers
AU  - Manik Chandra Barman
AU  - Srabone Sarker
AU  - Atikur Rahaman
AU  - Syed Ali Azmal
AU  - Jannatun Nime
AU  - Abdus Sattar
AU  - Akramul Haque
AU  - Shiekh Mohammad Abdul Matin
AU  - Abdul Hamid
Y1  - 2023/03/16
PY  - 2023
N1  - https://doi.org/10.11648/j.ijgg.20231101.14
DO  - 10.11648/j.ijgg.20231101.14
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 27
EP  - 37
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20231101.14
AB  - Forty-eight crossbreed heifers were artificially inseminated within 10-20 hours after standing heat to evaluate the effects of different doses of hormones and age on reproductive performance synchronized with GnRH and PGF2α. On day 90 after artificial insemination, the conception rate was confirmed by rectal palpation. There were significant differences (PPPPPPPPP>0.05) effect on conception rate by the onset of oesturs and duration of oesturs. The highest oesturs rate and conception rate was observed at 75.00% and 66.67%; respectively by administration of maximum doses of the hormone of body condition score (3) 69.23% of crossbreed heifer. When 1.5 ml GnRH was used in group III, the conception rate was observed higher at 25-30 months of age (77.78%) which were inseminated in the site of the deep cervix (77.77%) with frozen-thaw semen by experience inseminator at 10-14 hours (75%) after the onset of oesturs and naturally retrieve of oesturs on conception rate (75%). It was concluded that the age of the heifers, doses of the hormone, Body Condition Score (BCS), time of initiation of synchronization protocol, time of AI, semen placement and retrieve to oesturs naturally were the most important factors to get maximum oesturs response and conception rate of crossbreed heifers. So, it may be suggested that to achieve the desired rate of oesturs and conception, the farmers should inseminate their crossbreed heifers (BCS, 3 out of 5) with maximum doses of hormone in the deep cervix by technically sound and skilled inseminator at 10-14 hours after onset of oesturs in morning synchronization protocol. GnRH and PGF2α analogs could successfully induce oesturs response and improve the fertility of a dairy herd. Therefore, ovsynch protocol for crossbreed heifers is needed for further work involving a large number of animals for a definite conclusion.
VL  - 11
IS  - 1
ER  -

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Author Information

Manik Chandra Barman

Department of General Animal Science and Nutrition, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh
Srabone Sarker

Department of Political Science, Kurigram Govt. College, Kurigram, Bangladesh
Atikur Rahaman

Department of Biochemistry, Physiology & Pharmacology, Sirajganj Government Veterinary College, Sirajganj, Bangladesh
Syed Ali Azmal

Department of Genetics and Animal Breeding, Sirajganj Government Veterinary College, Sirajganj, Bangladesh
Jannatun Nime

Department of Microbiology, Sirajganj Government Veterinary College, Sirajganj, Bangladesh
Abdus Sattar

Department of Physiology, Bangladesh Agricultural University, Mymensingh, Bangladesh
Akramul Haque

Department of Animal Science and Nutrition, Sirajganj Government Veterinary College, Sirajganj, Bangladesh
Shiekh Mohammad Abdul Matin

Department of Medicine and Surgery, Sirajganj Government Veterinary College, Sirajganj, Bangladesh
Abdul Hamid

Department of General Animal Science and Nutrition, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

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Manik Chandra Barman, Srabone Sarker, Atikur Rahaman, Syed Ali Azmal, Jannatun Nime, et al. (2023). Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers. International Journal of Genetics and Genomics, 11(1), 27-37. https://doi.org/10.11648/j.ijgg.20231101.14

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ACS Style

Manik Chandra Barman; Srabone Sarker; Atikur Rahaman; Syed Ali Azmal; Jannatun Nime, et al. Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers. Int. J. Genet. Genomics 2023, 11(1), 27-37. doi: 10.11648/j.ijgg.20231101.14

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AMA Style

Manik Chandra Barman, Srabone Sarker, Atikur Rahaman, Syed Ali Azmal, Jannatun Nime, et al. Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers. Int J Genet Genomics. 2023;11(1):27-37. doi: 10.11648/j.ijgg.20231101.14

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@article{10.11648/j.ijgg.20231101.14,
  author = {Manik Chandra Barman and Srabone Sarker and Atikur Rahaman and Syed Ali Azmal and Jannatun Nime and Abdus Sattar and Akramul Haque and Shiekh Mohammad Abdul Matin and Abdul Hamid},
  title = {Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {27-37},
  doi = {10.11648/j.ijgg.20231101.14},
  url = {https://doi.org/10.11648/j.ijgg.20231101.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.14},
  abstract = {Forty-eight crossbreed heifers were artificially inseminated within 10-20 hours after standing heat to evaluate the effects of different doses of hormones and age on reproductive performance synchronized with GnRH and PGF2α. On day 90 after artificial insemination, the conception rate was confirmed by rectal palpation. There were significant differences (PPPPPPPPP>0.05) effect on conception rate by the onset of oesturs and duration of oesturs. The highest oesturs rate and conception rate was observed at 75.00% and 66.67%; respectively by administration of maximum doses of the hormone of body condition score (3) 69.23% of crossbreed heifer. When 1.5 ml GnRH was used in group III, the conception rate was observed higher at 25-30 months of age (77.78%) which were inseminated in the site of the deep cervix (77.77%) with frozen-thaw semen by experience inseminator at 10-14 hours (75%) after the onset of oesturs and naturally retrieve of oesturs on conception rate (75%). It was concluded that the age of the heifers, doses of the hormone, Body Condition Score (BCS), time of initiation of synchronization protocol, time of AI, semen placement and retrieve to oesturs naturally were the most important factors to get maximum oesturs response and conception rate of crossbreed heifers. So, it may be suggested that to achieve the desired rate of oesturs and conception, the farmers should inseminate their crossbreed heifers (BCS, 3 out of 5) with maximum doses of hormone in the deep cervix by technically sound and skilled inseminator at 10-14 hours after onset of oesturs in morning synchronization protocol. GnRH and PGF2α analogs could successfully induce oesturs response and improve the fertility of a dairy herd. Therefore, ovsynch protocol for crossbreed heifers is needed for further work involving a large number of animals for a definite conclusion.},
 year = {2023}
}

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TY  - JOUR
T1  - Effect of Age and Different Doses of GnRH and PGF2α Analogue on Oesturs Synchronization and Conception Rate of Crossbreed Heifers
AU  - Manik Chandra Barman
AU  - Srabone Sarker
AU  - Atikur Rahaman
AU  - Syed Ali Azmal
AU  - Jannatun Nime
AU  - Abdus Sattar
AU  - Akramul Haque
AU  - Shiekh Mohammad Abdul Matin
AU  - Abdul Hamid
Y1  - 2023/03/16
PY  - 2023
N1  - https://doi.org/10.11648/j.ijgg.20231101.14
DO  - 10.11648/j.ijgg.20231101.14
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 27
EP  - 37
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20231101.14
AB  - Forty-eight crossbreed heifers were artificially inseminated within 10-20 hours after standing heat to evaluate the effects of different doses of hormones and age on reproductive performance synchronized with GnRH and PGF2α. On day 90 after artificial insemination, the conception rate was confirmed by rectal palpation. There were significant differences (PPPPPPPPP>0.05) effect on conception rate by the onset of oesturs and duration of oesturs. The highest oesturs rate and conception rate was observed at 75.00% and 66.67%; respectively by administration of maximum doses of the hormone of body condition score (3) 69.23% of crossbreed heifer. When 1.5 ml GnRH was used in group III, the conception rate was observed higher at 25-30 months of age (77.78%) which were inseminated in the site of the deep cervix (77.77%) with frozen-thaw semen by experience inseminator at 10-14 hours (75%) after the onset of oesturs and naturally retrieve of oesturs on conception rate (75%). It was concluded that the age of the heifers, doses of the hormone, Body Condition Score (BCS), time of initiation of synchronization protocol, time of AI, semen placement and retrieve to oesturs naturally were the most important factors to get maximum oesturs response and conception rate of crossbreed heifers. So, it may be suggested that to achieve the desired rate of oesturs and conception, the farmers should inseminate their crossbreed heifers (BCS, 3 out of 5) with maximum doses of hormone in the deep cervix by technically sound and skilled inseminator at 10-14 hours after onset of oesturs in morning synchronization protocol. GnRH and PGF2α analogs could successfully induce oesturs response and improve the fertility of a dairy herd. Therefore, ovsynch protocol for crossbreed heifers is needed for further work involving a large number of animals for a definite conclusion.
VL  - 11
IS  - 1
ER  -

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