Study of the Genetic Diversity of Tiger Nut (<i>Cyperus esculentus</i>) Ecotypes of Niger Using SSR Markers

Bori Haoua; Idi Saidou Sani; Zangui Hamissou; Adam Toudou

doi:doi:10.11648/j.ijgg.20231101.13

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Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers

Bori Haoua, Idi Saidou Sani, Zangui Hamissou, Adam Toudou

Published in International Journal of Genetics and Genomics (Volume 11, Issue 1)

Received: 9 February 2023 Accepted: 25 February 2023 Published: 9 March 2023

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Abstract

Tiger Nuts (Cyperus esculentus L.), a member of the Cyperaceae family, is a plant cultivated in Niger for its tubers. The present study focuses on the molecular characterization of 10 Tiger Nuts (Cyperus esculentus L.) ecotypes from Niger. These Tiger Nuts ecotypes were collected in the Tiger Nuts producing regions of Niger (Dosso and Maradi). DNA extraction and genetic diversity analysis were carried out at the molecular biology laboratories of ICRISAT, Hyderabad, India. Codominant nuclear and polymorphic microsatellite (SSR) markers were used. The genetic diversity parameters calculated are: polymorphism rate (P) at the 95% threshold, allelic diversity (A), observed (Ho) and expected (He) heterozygosity rate and the panmixy deviation in a subpopulation (Fis) under the Hardy-Weinberg hypotheses. The results showed that genetic diversity ranged from 0.03 (StvCyR_181a and StvCyR_327ska) to 0.60 (StvCyR_93ska) with a mean of 0.28 and. For the Tiger Nuts producing regions, the average deficit in heterozygotes is highly significant, with a mean Fis of 0.415 (Fis, 1000 permutation test, P<0.001). A significant amount of variability (27%) from differentiation between the ecotypes studied was observed in the analysis of molecular variance. These results clearly show a genetic differentiation between the populations of the large, small and wild Tiger Nuts, grouping them into three distinct groups. The study also showed that the genetic structure of the Tiger Nuts is not linked to a particular geographical origin and that the molecular tests give us evidence of conserved variability in the Niger Tiger Nuts ecotypes.

Published in	International Journal of Genetics and Genomics (Volume 11, Issue 1)
DOI	10.11648/j.ijgg.20231101.13
Page(s)	18-26
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Tiger Nuts (Cyperus esculentus L.), Ecotypes, Genetic Diversity, SSR Markers, Regions, Niger

References

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APA Style

Bori Haoua, Idi Saidou Sani, Zangui Hamissou, Adam Toudou. (2023). Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers. International Journal of Genetics and Genomics, 11(1), 18-26. https://doi.org/10.11648/j.ijgg.20231101.13

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ACS Style

Bori Haoua; Idi Saidou Sani; Zangui Hamissou; Adam Toudou. Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers. Int. J. Genet. Genomics 2023, 11(1), 18-26. doi: 10.11648/j.ijgg.20231101.13

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AMA Style

Bori Haoua, Idi Saidou Sani, Zangui Hamissou, Adam Toudou. Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers. Int J Genet Genomics. 2023;11(1):18-26. doi: 10.11648/j.ijgg.20231101.13

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@article{10.11648/j.ijgg.20231101.13,
  author = {Bori Haoua and Idi Saidou Sani and Zangui Hamissou and Adam Toudou},
  title = {Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {18-26},
  doi = {10.11648/j.ijgg.20231101.13},
  url = {https://doi.org/10.11648/j.ijgg.20231101.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.13},
  abstract = {Tiger Nuts (Cyperus esculentus L.), a member of the Cyperaceae family, is a plant cultivated in Niger for its tubers. The present study focuses on the molecular characterization of 10 Tiger Nuts (Cyperus esculentus L.) ecotypes from Niger. These Tiger Nuts ecotypes were collected in the Tiger Nuts producing regions of Niger (Dosso and Maradi). DNA extraction and genetic diversity analysis were carried out at the molecular biology laboratories of ICRISAT, Hyderabad, India. Codominant nuclear and polymorphic microsatellite (SSR) markers were used. The genetic diversity parameters calculated are: polymorphism rate (P) at the 95% threshold, allelic diversity (A), observed (Ho) and expected (He) heterozygosity rate and the panmixy deviation in a subpopulation (Fis) under the Hardy-Weinberg hypotheses. The results showed that genetic diversity ranged from 0.03 (StvCyR_181a and StvCyR_327ska) to 0.60 (StvCyR_93ska) with a mean of 0.28 and. For the Tiger Nuts producing regions, the average deficit in heterozygotes is highly significant, with a mean Fis of 0.415 (Fis, 1000 permutation test, P<0.001). A significant amount of variability (27%) from differentiation between the ecotypes studied was observed in the analysis of molecular variance. These results clearly show a genetic differentiation between the populations of the large, small and wild Tiger Nuts, grouping them into three distinct groups. The study also showed that the genetic structure of the Tiger Nuts is not linked to a particular geographical origin and that the molecular tests give us evidence of conserved variability in the Niger Tiger Nuts ecotypes.},
 year = {2023}
}

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TY  - JOUR
T1  - Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers
AU  - Bori Haoua
AU  - Idi Saidou Sani
AU  - Zangui Hamissou
AU  - Adam Toudou
Y1  - 2023/03/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ijgg.20231101.13
DO  - 10.11648/j.ijgg.20231101.13
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 18
EP  - 26
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20231101.13
AB  - Tiger Nuts (Cyperus esculentus L.), a member of the Cyperaceae family, is a plant cultivated in Niger for its tubers. The present study focuses on the molecular characterization of 10 Tiger Nuts (Cyperus esculentus L.) ecotypes from Niger. These Tiger Nuts ecotypes were collected in the Tiger Nuts producing regions of Niger (Dosso and Maradi). DNA extraction and genetic diversity analysis were carried out at the molecular biology laboratories of ICRISAT, Hyderabad, India. Codominant nuclear and polymorphic microsatellite (SSR) markers were used. The genetic diversity parameters calculated are: polymorphism rate (P) at the 95% threshold, allelic diversity (A), observed (Ho) and expected (He) heterozygosity rate and the panmixy deviation in a subpopulation (Fis) under the Hardy-Weinberg hypotheses. The results showed that genetic diversity ranged from 0.03 (StvCyR_181a and StvCyR_327ska) to 0.60 (StvCyR_93ska) with a mean of 0.28 and. For the Tiger Nuts producing regions, the average deficit in heterozygotes is highly significant, with a mean Fis of 0.415 (Fis, 1000 permutation test, P<0.001). A significant amount of variability (27%) from differentiation between the ecotypes studied was observed in the analysis of molecular variance. These results clearly show a genetic differentiation between the populations of the large, small and wild Tiger Nuts, grouping them into three distinct groups. The study also showed that the genetic structure of the Tiger Nuts is not linked to a particular geographical origin and that the molecular tests give us evidence of conserved variability in the Niger Tiger Nuts ecotypes.
VL  - 11
IS  - 1
ER  -

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Author Information

Bori Haoua

Irrigated Crops Department, National Institute of Agronomic Research of Niger, Niamey, Niger
Idi Saidou Sani

Faculty of Agronomic Science, University of Diffa, Diffa, Niger
Zangui Hamissou

Faculty of Agronomy, Abdou Moumouni University, Niamey, Niger
Adam Toudou

Faculty of Agronomy, Abdou Moumouni University, Niamey, Niger

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Plain Text BibTeX RIS

APA Style

Bori Haoua, Idi Saidou Sani, Zangui Hamissou, Adam Toudou. (2023). Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers. International Journal of Genetics and Genomics, 11(1), 18-26. https://doi.org/10.11648/j.ijgg.20231101.13

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ACS Style

Bori Haoua; Idi Saidou Sani; Zangui Hamissou; Adam Toudou. Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers. Int. J. Genet. Genomics 2023, 11(1), 18-26. doi: 10.11648/j.ijgg.20231101.13

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AMA Style

Bori Haoua, Idi Saidou Sani, Zangui Hamissou, Adam Toudou. Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers. Int J Genet Genomics. 2023;11(1):18-26. doi: 10.11648/j.ijgg.20231101.13

Copy | Download

@article{10.11648/j.ijgg.20231101.13,
  author = {Bori Haoua and Idi Saidou Sani and Zangui Hamissou and Adam Toudou},
  title = {Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {18-26},
  doi = {10.11648/j.ijgg.20231101.13},
  url = {https://doi.org/10.11648/j.ijgg.20231101.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.13},
  abstract = {Tiger Nuts (Cyperus esculentus L.), a member of the Cyperaceae family, is a plant cultivated in Niger for its tubers. The present study focuses on the molecular characterization of 10 Tiger Nuts (Cyperus esculentus L.) ecotypes from Niger. These Tiger Nuts ecotypes were collected in the Tiger Nuts producing regions of Niger (Dosso and Maradi). DNA extraction and genetic diversity analysis were carried out at the molecular biology laboratories of ICRISAT, Hyderabad, India. Codominant nuclear and polymorphic microsatellite (SSR) markers were used. The genetic diversity parameters calculated are: polymorphism rate (P) at the 95% threshold, allelic diversity (A), observed (Ho) and expected (He) heterozygosity rate and the panmixy deviation in a subpopulation (Fis) under the Hardy-Weinberg hypotheses. The results showed that genetic diversity ranged from 0.03 (StvCyR_181a and StvCyR_327ska) to 0.60 (StvCyR_93ska) with a mean of 0.28 and. For the Tiger Nuts producing regions, the average deficit in heterozygotes is highly significant, with a mean Fis of 0.415 (Fis, 1000 permutation test, P<0.001). A significant amount of variability (27%) from differentiation between the ecotypes studied was observed in the analysis of molecular variance. These results clearly show a genetic differentiation between the populations of the large, small and wild Tiger Nuts, grouping them into three distinct groups. The study also showed that the genetic structure of the Tiger Nuts is not linked to a particular geographical origin and that the molecular tests give us evidence of conserved variability in the Niger Tiger Nuts ecotypes.},
 year = {2023}
}

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TY  - JOUR
T1  - Study of the Genetic Diversity of Tiger Nut (Cyperus esculentus) Ecotypes of Niger Using SSR Markers
AU  - Bori Haoua
AU  - Idi Saidou Sani
AU  - Zangui Hamissou
AU  - Adam Toudou
Y1  - 2023/03/09
PY  - 2023
N1  - https://doi.org/10.11648/j.ijgg.20231101.13
DO  - 10.11648/j.ijgg.20231101.13
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 18
EP  - 26
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20231101.13
AB  - Tiger Nuts (Cyperus esculentus L.), a member of the Cyperaceae family, is a plant cultivated in Niger for its tubers. The present study focuses on the molecular characterization of 10 Tiger Nuts (Cyperus esculentus L.) ecotypes from Niger. These Tiger Nuts ecotypes were collected in the Tiger Nuts producing regions of Niger (Dosso and Maradi). DNA extraction and genetic diversity analysis were carried out at the molecular biology laboratories of ICRISAT, Hyderabad, India. Codominant nuclear and polymorphic microsatellite (SSR) markers were used. The genetic diversity parameters calculated are: polymorphism rate (P) at the 95% threshold, allelic diversity (A), observed (Ho) and expected (He) heterozygosity rate and the panmixy deviation in a subpopulation (Fis) under the Hardy-Weinberg hypotheses. The results showed that genetic diversity ranged from 0.03 (StvCyR_181a and StvCyR_327ska) to 0.60 (StvCyR_93ska) with a mean of 0.28 and. For the Tiger Nuts producing regions, the average deficit in heterozygotes is highly significant, with a mean Fis of 0.415 (Fis, 1000 permutation test, P<0.001). A significant amount of variability (27%) from differentiation between the ecotypes studied was observed in the analysis of molecular variance. These results clearly show a genetic differentiation between the populations of the large, small and wild Tiger Nuts, grouping them into three distinct groups. The study also showed that the genetic structure of the Tiger Nuts is not linked to a particular geographical origin and that the molecular tests give us evidence of conserved variability in the Niger Tiger Nuts ecotypes.
VL  - 11
IS  - 1
ER  -

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